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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, J. A.; RIBEIRO, J. B.; GONÇALVES, D. B.; QUEIROZ, M. V. de; ARAÚJO, E. F. de. |
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Afiliação: |
JANAINA APARECIDA TEIXEIRA, UFV; JOAO BATISTA RIBEIRO, CNPGL; DANIEL BONOTO GONÇALVES, UFSJ; MARISA VIEIRA DE QUEIROZ, UFV; ELZA FERNANDES DE ARAÚJO, UFV. |
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Título: |
Overproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Applied Biochemistry and Biotechnology, v. 169, n. 6, p. 1965-1977, 2013. |
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DOI: |
https://doi.org/10.1007/s12010-013-0121-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Inactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector). This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum. The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production. |
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Palavras-Chave: |
Gene inactivation; Gpd promoter; Overproduction; Penicillium griseoroseum. |
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Thesaurus Nal: |
polygalacturonase. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02088naa a2200241 a 4500
001 1978792
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12010-013-0121-6$2DOI
100 1 $aTEIXEIRA, J. A.
245 $aOverproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene.$h[electronic resource]
260 $c2013
520 $aInactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector). This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum. The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production.
650 $apolygalacturonase
653 $aGene inactivation
653 $aGpd promoter
653 $aOverproduction
653 $aPenicillium griseoroseum
700 1 $aRIBEIRO, J. B.
700 1 $aGONÇALVES, D. B.
700 1 $aQUEIROZ, M. V. de
700 1 $aARAÚJO, E. F. de
773 $tApplied Biochemistry and Biotechnology$gv. 169, n. 6, p. 1965-1977, 2013.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 10 | |
| 4. |  | SANTANA, M. F.; SILVA, J. C. F.; BATISTA, A. D.; RIBEIRO, L. E.; SILVA, G. F. da; ARAÚJO, E. F. de; QUEIROZ, M. V. de. Abundance, distribution and potential impact of transposable elements in the genome of Mycosphaerella fijiensis. BMC Genomics, v. 13, n. 1, p. 1-11, Dec. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 5. | | ABADIO, A. K. R.; LIMA, S. S.; SANTANA, M. F.; SALOMÃO, T. M. F.; SARTORATO, A.; MIZUBUTI, E. S. G.; ARAÚJO, E. F.; QUEIROZ, M. V. de. Genetic diversity analysis of isolates of the fungal bean pathogen Pseudocercospora griseola from central and southern Brazil. Genetics and Molecular Research, v. 11, n. 2, p. 1272-1279, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 6. | | BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de. Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola. Mycologia, New York, v. 106, n. 4, p. 629-641, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | PEREIRA, J. F.; ALMEIDA, A. P. M. M.; COTA, J.; PAMPHILE, J. A.; SILVA, G. F. da; ARAUJO, E. F. de; GRAMACHO, K. P.; BROMMONSCHENKEL, S. H.; PEREIRA, G. A. G.; QUEIROZ, M. V. de. Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus. Microbiology, Reading, v. 159, p. 112-125, Jan. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Trigo. |
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| 9. | | SOUSA, T. F.; ARAÚJO JÚNIOR, M. B. de; PERES, E. G.; SOUZA, M. P.; SILVA, F. M. A. da; MEDEIROS, L. S. de; SOUZA, A. D. L. de; SOUZA, A. Q. L. de S.; YAMAGISHI, M. E. B.; SILVA, G. F. da; KOOLEN, H. H. F.; QUEIROZ, M. V. de. Discovery of dual PKS involved in sclerotiorin biosynthesis in Penicillium meliponae using genome mining and gene knockout. Archives of Microbiology, v. 205, n. 2, 75, Feb. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
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| 10. | | GUIMARÃES, S. da S. C.; TAVARES, D. G.; MONTEIRO, M. C. P.; PEDROSO, M. P.; NUNES, C. A.; MOURÃO, B.; CARVALHO, I. S. e; BARDAJÍ, D. K. R.; CAMARGO, I. L. B. C.; LANA, U. G. de P.; GOMES, E. A.; QUEIROZ, M. V. de; PEREIRA, O. L.; CARDOSO, P. G. Polyphasic characterization and antimicrobial properties of Induratia species isolated from Cofea arabica in Brazil. Mycological Progress, v. 20, p. 1457-1477, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 10 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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